Eight years of single-molecule localization microscopy
نویسندگان
چکیده
منابع مشابه
Practical considerations for single molecule localization microscopy
This is an especially exciting era in biological optical microscopy, a series of new and accessible far-field ‘superresolution’ fluorescence techniques are changing the way we view cells. These techniques surpass the traditional resolution limit dictated by the diffraction of light, approaching levels previously occupied solely by electron microscopy. Herein the authors review and discuss some ...
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Single molecule localization microscopy (SMLM), which can provide up to an order of magnitude improvement in spatial resolution over conventional fluorescence microscopy, has the potential to be a highly useful tool for quantitative biological experiments. It has already been used for this purpose in varied fields in biology, ranging from molecular biology to neuroscience. In this review articl...
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Localization of single molecules in microscopy images is a key step in quantitative single particle data analysis. Among them, single molecule based super-resolution optical microscopy techniques require high localization accuracy as well as computation of large data sets in the order of 10(5) single molecule detections to reconstruct a single image. We hereby present an algorithm based on imag...
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One of the most basic questions in single-molecule microscopy concerns the accuracy with which the location of a single molecule can be determined. Using the Fisher information matrix it is shown that the limit of the localization accuracy for a single molecule is given by, lambda(em)/2pi n(a) square root of gammaAt, where lambda(em), n(a), gamma, A, and t denote the emission wavelength of the ...
متن کاملEffects of fixed pattern noise on single molecule localization microscopy.
The newly developed scientific complementary metal oxide semiconductor (sCMOS) cameras are capable of realizing fast single molecule localization microscopy without sacrificing field-of-view, benefiting from their readout speed which is significantly higher than that of conventional charge-coupled device (CCD) cameras. However, the poor image uniformity (suffered from fixed pattern noise, FPN) ...
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ژورنال
عنوان ژورنال: Histochemistry and Cell Biology
سال: 2014
ISSN: 0948-6143,1432-119X
DOI: 10.1007/s00418-014-1184-3